Journal: Respiratory Research

Article Title: Gene profile of fibroblasts identify relation of CCL8 with idiopathic pulmonary fibrosis

doi: 10.1186/s12931-016-0493-6

Figure Lengend Snippet: Genes showing 10-fold or greater changes in gene expression between the IPF and control groups

Article Snippet: In addition, we measured the CCL8 protein amount in supernatants and cell lysates of the cultured fibroblasts (1 × 10 6 ) using CCL8 ELISA kit (Abnova, Taipei, Taiwan).

Techniques: Gene Expression, Control

Journal: Respiratory Research

Article Title: Gene profile of fibroblasts identify relation of CCL8 with idiopathic pulmonary fibrosis

doi: 10.1186/s12931-016-0493-6

Figure Lengend Snippet: CCL8 mRNA and protein levels in lung tissue-derived fibroblasts from 14 IPF patients and 10 controls. ( a ) RT-PCR, ( b ) densitometry of the CCL8 RT-PCR band intensity normalized to that of β-actin, ( c ) real-time PCR, and ( d ) correlations of the CCL8 mRNA levels of 12 subjects determined by the transcriptome chip with those by real-time PCR. e , f CCL8 protein level of Culture media and cell lysate, and ( g ) correlations of the CCL8 protein levels and CCL8 mRNA levels of 24 subjects determined by the ELISA with those by real-time PCR. The data were presented as median values with 25 and 75% quartiles

Article Snippet: In addition, we measured the CCL8 protein amount in supernatants and cell lysates of the cultured fibroblasts (1 × 10 6 ) using CCL8 ELISA kit (Abnova, Taipei, Taiwan).

Techniques: Derivative Assay, Reverse Transcription Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

Journal: Respiratory Research

Article Title: Gene profile of fibroblasts identify relation of CCL8 with idiopathic pulmonary fibrosis

doi: 10.1186/s12931-016-0493-6

Figure Lengend Snippet: CCL8 protein concentrations in BAL fluids and ROC curves. a CCL8 protein was detected in 25 of 41 normal controls, 80 of 86 IPF patients, 11 of 22 NSIP patients, 8 of 20 HP patients and 13 of 19 sarcoidosis patients. Open and closed circles indicate CCL8 protein levels detected (>1.5pg/mL) and those below the lower limit of detection, respectively. The data were presented as median values with 25 and 75% quartiles. b ROC curve of the CCL8 protein concentration between the two groups. A cut-off value of 2.17pg/mL had 80.2% accuracy, 86.0% specificity, and 65.7% sensitivity for differentiating IPF patients from controls. c ROC curve of the CCL8 protein concentration between the other interstitial lung diseases groups. A cut-off value 0.53pg/mL had 70.7% accuracy, 91.9% specificity, 57.4% sensitivity between the IPF patients and the other interstitial lung diseases group. d A Kaplan-Meier plot of 69 subjects with IPF followed up for 1to 8 years. The percent survival rate was markedly lower in the group with a CCL8 level of >28.61 pg/mL (red line) compared with that in the group with a CCL8 level of <28.61 pg/mL (black line) (hazard ratio = 3.93, CI: 1.25–12.39, p = 0.012)

Article Snippet: In addition, we measured the CCL8 protein amount in supernatants and cell lysates of the cultured fibroblasts (1 × 10 6 ) using CCL8 ELISA kit (Abnova, Taipei, Taiwan).

Techniques: Protein Concentration

Journal: Respiratory Research

Article Title: Gene profile of fibroblasts identify relation of CCL8 with idiopathic pulmonary fibrosis

doi: 10.1186/s12931-016-0493-6

Figure Lengend Snippet: Representative double immunofluorescence-stained images of IPF and control lung tissues. CCL8 and α-smooth muscle actin (α-SMA) were stained using PE- (red) and FITC-conjugated antibodies (green), respectively. A proportion of interstitial fibroblasts (IT) and the peribronchial and vascular area (VS) showed staining for both CCL8 and α-SMA (magnification, 200×)

Article Snippet: In addition, we measured the CCL8 protein amount in supernatants and cell lysates of the cultured fibroblasts (1 × 10 6 ) using CCL8 ELISA kit (Abnova, Taipei, Taiwan).

Techniques: Immunofluorescence, Staining, Control

Journal: Molecules

Article Title: Beneficial Effects of Naringenin in Cigarette Smoke-Induced Damage to the Lung Based on Bioinformatic Prediction and In Vitro Analysis

doi: 10.3390/molecules25204704

Figure Lengend Snippet: The effects of naringenin on CSE-induced IL-8 ( A ) and TNF-α ( B ) levels in BEAS-2B cells. Data are expressed as the means ± SD. ** p < 0.01 vs. Control group; # p < 0.05, ## p < 0.01 vs. CSE alone group.

Article Snippet: The ELISA kits for IL-8 and TNF-α were purchased from USCNK (Wuhan, China).

Techniques:

Journal: PLoS ONE

Article Title: Antiviral Activity and Possible Mechanism of Action of Constituents Identified in Paeonia lactiflora Root toward Human Rhinoviruses

doi: 10.1371/journal.pone.0121629

Figure Lengend Snippet: mRNA expressions of interleukin (IL)-6 ( A ), IL-8 ( B ), tumor necrosis factor (TNF) ( C ), interferon beta (IFN-β) ( D ), and IL-1β ( E ) were detected by real-time quantitative reverse transcription-PCR in MRC5 2 days after infection in the presence of 100 μg/mL paeonol (PA) or 20 μg/mL 1,2,3,4,6-penta- O -galloyl-β-D-glucopyranose (PGG). Each bar represents the mean ± SD of triplicate samples of three independent experiments. ***p<0.001, **p<0.01, using a Bonferroni multiple comparison post-test.

Article Snippet: OptEIA IL-6, IL-8, and TNF ELISA kits (BD Biosciences, San Diego, CA) were used for the assays.

Techniques: Infection

Journal: PLoS ONE

Article Title: Antiviral Activity and Possible Mechanism of Action of Constituents Identified in Paeonia lactiflora Root toward Human Rhinoviruses

doi: 10.1371/journal.pone.0121629

Figure Lengend Snippet: Concentrations of interleukin (IL)-6 ( A ) and IL-8 ( B ) was detected by ELISA in MRC5 supernatants after 2 day infection in the presence of 100 μg/mL paeonol (PA) and 20 μg/mL 1,2,3,4,6-penta- O -galloyl-β-D-glucopyranose (PGG). Each bar represents the mean ± SD of triplicate samples of three independent experiments. ***p<0.001, **p<0.01, using a Bonferroni multiple comparison post-test.

Article Snippet: OptEIA IL-6, IL-8, and TNF ELISA kits (BD Biosciences, San Diego, CA) were used for the assays.

Techniques: Enzyme-linked Immunosorbent Assay, Infection

Journal: NPJ Aging and Mechanisms of Disease

Article Title: Clampdown of inflammation in aging and anticancer therapies by limiting upregulation and activation of GPCR, CXCR4

doi: 10.1038/s41514-018-0028-0

Figure Lengend Snippet: CXCR4 receptor regulates DNA-damage associated inflammation. For all experiments, media from treated cells (as indicated), was used for sandwich ELISA for determining levels of IL8 or IL6 pg/ml secreted per 10 3 cells, represented as fold change over control untreated cells. a Effect of activation of CXCR4-CXCL12 signaling during DNA damage on IL8 and IL6 cytokine secretion ( n > 6). b Effect of CXCR4 inhibition (AMD treatment) on IL6 production from senescent cells post-CXCL12 stimulation. Cells were treated with various compounds as indicated and IL6 levels in supernatant were analysed ( n = 3). c Effect of inhibition of Gαi by PTx treatment. Comparison of IL8 levels between HeLa cells treated with BrdU; BrdU + CXCL12 or BrdU + CXCL12 in presence of pertussis toxin (PTx) ( n = 3). d Effect of inhibition of Gαi by PTx treatment on MRC5 cells. Comparison of IL8 levels in MRC5, primary cells treated with BrdU; BrdU + CXCL12 and BrdU + CXCL12 in presence of PTx ( n = 3). For all experiments, results are represented as mean ± s.e.m. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.001 (Student’s t -test)

Article Snippet: For estimating extracellular levels of various cytokines using media collected from treated cells as indicated, BD OptiEIA™ Human IL8, IL6 and TGFβ ELISA kits (BD Biosciences, USA) were used as per manufacturer’s instructions.

Techniques: Sandwich ELISA, Activation Assay, Inhibition

Journal: Iranian Journal of Basic Medical Sciences

Article Title: 6-O-angeloylplenolin inhibits anti-IgE-stimulated human mast cell activation via suppressing calcium influx and ERK phosphorylation

doi: 10.22038/IJBMS.2022.64132.14120

Figure Lengend Snippet: Histamine and pro-inflammatory cytokines released by human mast cells HCMC and LAD2 were inhibited by 6-O-angeloylplenolin (6-OAP). Sensitized mast cells were pre-incubated with 6-OAP for 8 hr and then stimulated with 1 µg/ml anti-IgE for promoting degranulation and cytokine release. The effects of various concentrations of 6-OAP on degranulation as indicated by histamine release (a), Interleukin-8 (IL-8) release (b), and Tumor necrosis factor-α (TNF-α) release (c) for LAD2 and human cultured mast cell (HCMC) are listed. All results were corrected for basal release. It is found that 6-OAP significantly suppressed the release of histamine, IL-8, and TNF-α in a dose-dependent manner in both LAD2 and HCMC cells. Data are presented as means ± SEM. * P <0.05 and ** P <0.01 by one-way ANOVA

Article Snippet: ELISA kits for interleukin-8 (IL-8) and tumor necrosis factor-α (TNF-α) were purchased from BD Biosciences (NJ, USA).

Techniques: Incubation, Cell Culture

Journal: Toxicology Reports

Article Title: Emissions from commercial-grade charbroiling meat operations induce oxidative stress and inflammatory responses in human bronchial epithelial cells

doi: 10.1016/j.toxrep.2014.09.015

Figure Lengend Snippet: OE elevated the levels of IL-6 (A) and IL-8 (B) in BEAS-2B cell culture media. Cells were exposed to OE at indicated concentration for 16 h. Values represent means ± SEM, n = 3, * p < 0.05 compared to the respective control.

Article Snippet: OptEIA™ Human IL-6 and IL-8 ELISA kits and type I rat tail collagen were purchased from BD Bioscience (San Diego, CA).

Techniques: Cell Culture, Concentration Assay

Journal: Toxicology Reports

Article Title: Emissions from commercial-grade charbroiling meat operations induce oxidative stress and inflammatory responses in human bronchial epithelial cells

doi: 10.1016/j.toxrep.2014.09.015

Figure Lengend Snippet: Inhibition of OE-induced IL-6 and IL-8 production by NAC and SFN. BEAS-2B cells were pre-incubated with or without NAC or SFN for 2 h before exposure to OE for 16 h. (A and B) Effects of NAC and SFN on IL-6. (C and D) Effects of NAC and SFN on IL-8. Inset: Induction of HO-1 expression by SFN after a 6-h exposure. OE50: OE at 50 μg/ml. Values represent means ± SEM, n = 3. * p < 0.05 compared to the respective control; ** p < 0.05 compared to OE without NAC.

Article Snippet: OptEIA™ Human IL-6 and IL-8 ELISA kits and type I rat tail collagen were purchased from BD Bioscience (San Diego, CA).

Techniques: Inhibition, Incubation, Expressing

Journal: Toxicology Reports

Article Title: Emissions from commercial-grade charbroiling meat operations induce oxidative stress and inflammatory responses in human bronchial epithelial cells

doi: 10.1016/j.toxrep.2014.09.015

Figure Lengend Snippet: The role of p38 in mediating the pro-inflammatory effect of OE. (A) Increased p38 phosphorylation in OE-treated BEAS-2B cells. Cells were exposed to OE for 2 and 4 h. Total p38 was used as internal control. (B) Effect of p38 inhibitor on IL-6 production by OE-treated cells. (C). Effect of p38 inhibitor on OE-induced IL-8 production. BEAS-2B cells were pre-incubated with p38 inhibitor SB203580 for 2 h prior to being exposed to OE for 16 h. OE50: OE at 50 μg/ml. Values represent means ± SEM, n = 3, * p < 0.05 compared to the respective control; ** p < 0.05 compared to OE alone.

Article Snippet: OptEIA™ Human IL-6 and IL-8 ELISA kits and type I rat tail collagen were purchased from BD Bioscience (San Diego, CA).

Techniques: Incubation